Gingival Crevicular Fluid Flow Rate as an Indicator for Optimum Orthodontic Force: A Split-Mouth, Randomized Control Trial [RETRACTED]
Author(s): Harraa S Mohammed-Salih*, Hayder F Saloom
Objective: To investigate whether and when would gingival crevicular fluid (GCF) flow rate be affected by the magnitude of different orthodontic forces (OF). Materials and Methods: Eighteen adults, needed bilateral extraction of upper first premolars (UFPs) as part of their orthodontic treatment, were recruited. The right and left UFPs were randomly assigned to be subjected to either light (25g) or heavy (225g) force sectional fixed appliances. GCF was collected at four sites for each UFP at 0, 1hr, 1day, 7days, 14days, 21days and 28days of force application. In order to calibrate GCF flow rate, a Periotron 8010 was used to measure the GCF volume at each time point after force application and then it was compared to the reference time zero. The Freidman test as used to assess whether GCF flow rate was affected by the force magnitude at each time point. Statistical Analysis: Mixed-Design two-way repeated measures analysis of variance (ANOVA) was run to test the effect of force and time, and their interactions on GCF flow rate. This study was registered retrospectively in U.S. National Library of Medicine/ClinicalTrials.gov on March 2, 2020 with registration ID: NCT04291443. Results: Both force magnitudes showed significant reduction in GCF flow rate (P<0.05), with a sharp and clinically obvious decrease after 1hr of 225g force application. The light force showed a gradual reduction over the tested time points. Conclusions: GCF flow rate is sensitive to orthodontic force. It is negatively related. One-hour post force application is a good time for testing.