A Comparative Study on Antimicrobial Effect of Iranian Green Tea and Hibiscus Tea on Growth of Oral Cariogenic Bacteria Streptococcus mutans PTCC 1683
Author(s): AmirSina Golmohammadi
Abstract
Tooth caries is a major health problem today. Tea with its polyphenolic compounds can show its anticariogenic effects by affecting the main Cariogenic bacteria in the oral cavity. The present study tended to examine the antimicrobial effect of two Iranian teas Camellia sinensis and Hibiscus Sabdariffa in comparison with chlorhexidine on Iranian type of Cariogenic Streptococcus mutans Bacteria. For this purpose, ethanolic extract of plants was first processed; then, the impregnated disk was placed on the bacterial culture medium and diameter of inhibition zones was calculated. After three iterations, the results showed the means 17, 14.16 and 16.83 for zone of inhibition diameter of green tee, hibiscus tea, and control chlorhexidine 0.2%, respectively. Moreover, ANOVA test showed a significant difference between zones of inhibition diameter of Hibiscus Sabdariffa and Camellia sinensis. Multiple comparison tests showed no significant difference between zones of inhibition diameter of these two teas and chlorohexidine control. Examining minimum bactericidal concentration (MBC) and minimum inhibitory concentration (MIC) of both hibiscus and green teas also showed that MBC was present in 12.5 mg/ml green tea and 50 mg/ml hibiscus tea and MIC was present in 6.25 mg/ml green tea and 25 mg/ml hibiscus tea. According to findings, it is concluded that anti-microbial effects of Iranian green tea are higher than Iranian hibiscus tea. There is also no significant difference between antimicrobial effects of these three compounds. Accordingly, it is suggested to consider anti cariogenic effect of Iranian green tea and Iranian hibiscus tea and their daily intake.
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