Absence of Simian virus 40 DNA in salivary glands among Sudanese patients with Pleomorphic adenoma
Author(s): Asaad Mohammed, Dalia Mohammed, Ahmed Suleiman, Waleed Omer, Eltayib Saad
Background: Pleomorphic adenoma is a common benign salivary gland neoplasm characterized by neoplastic proliferation of parenchymatous glandular cells along with myoepithelial components, having a malignant potentiality is the commonest benign tumor to arise in the minor salivary gland. Simian virus 40 (SV40), is a highly oncogenic DNA tumor virus (polyomaviridae) which was recently found to be associated with various human tumors including pleomorphic adenoma. The virus is a monkey virus which was believed to be transmitted to humans only under exceptional situations.
Objective: The aim of this study was to evaluate the presence of SV40 DNA among Sudanese patient with pleomorphic adenoma of the salivary glands.
Material and Method: This is a case control study was conducted at Khartoum state, during period 2006-2012. Thirty study samples represents as cases that had salivary gland pleomorphic adenoma and ten normal salivary gland tissues represent as controls. Qualitative polymerase chain reaction (PCR) was used to evaluate the presence of SV40 DNA.
Results: In this study, human pleomorphic adenoma (PA) of salivary gland specimens and normal salivary gland tissues used as control were analyzed by qualitative polymerase chain reaction (PCR) for SV40. All sample (case and control) Tissue specimens were SV40 negative.
Conclusion: The findings of this study showed the absence of SV40 DNA in salivary and did not indicate any role among patients with PA.