Antibacterial Efficacy of Fenugreek Seed Extract Rinse against Streptococcus Mutants Colonies
Background: Antibiotics has been widely used as standard antibacterial treatment for long but exploited use of these drugs, has led microorganisms to developed resistance against many antibiotics. While 33% of the population are dependent on the therapeutic plant extract to meet their essential need, the awareness towards these phytochemicals extracts have gained wide acceptability and are being prescribed by the physicians as a treatment option for antibacterial medications. Potential source of new antibacterial compound which are sourced through extracts of fenugreek seeds was found to have effect on six different strains of pathological bacteria. The aim of current study is to further identify the antibacterial efficacy of fenugreek seed extract mouth rinse against streptococcus mutants. Methodology: It was a preclinical experimental study conducted at Shaheehd Muhtarma Benazir Bhutto Medical College, Lyari Larachi, Pakistan, from January - June 2021. Calculated sample size was n=60 Consecutive sampling technique was used. Plaque of study participants was collected on sterile strips that were transported to laboratory for culture in sterile containers. For culture S. mutant samples were inoculated in Columbia Agar with 5% sheep blood and incubated for 48 h at 37 °C and increased level of CO2. The fenugreek seed extract was diluted in distilled water in 1:4 (Fenugreek seed extract: Distilled water). Study participants were instructed to not brush their teeth before sampling. Study participants were divided into three groups (negative control, positive control and experimental) each group had 20 participants. Diluted Fenugreek seed extract was given to experimental group for rinses, distilled water was given to negative control group and Positive controls were given a commercially available mouth rinse. Results: Paired analysis depicted that there was significant decrease in number of colonies in positive controls and experimental group and there was no change in number of colonies in control group. Conclusion: Fenugreek seed extract showed comparable antibacterial properties to commercially available mouth rinse and reduced the number of colonies nearly equivalent to that.