Antibiotic susceptibility pattern of pseudomonas aeruginosa isolated at SSG hospital Baroda
Author(s): Hiral Patel, R.N.Garala
Background: Pseudomonas aeruginosa is one of the important bacterial pathogens isolated from various samples. Despite advances in medical and surgical care and introduction of wide variety of antimicrobial agents, Pseudomonas aeruginosa continues to cause life threatening infection & complications in hospital acquired infections.
Aims: To determine the antimicrobial susceptibility pattern of Pseudomonas aeruginosa.
Material and Method: This prospective study was conducted from January 2012 to June 2012. During these period total 6390 samples (blood, wound pus, sputum, various body fluids, urine) were tested, of which 4076 samples showed growth. Out of 4076, 415 Pseudomonas aeruginosa were isolated. The samples were selected on the basis of their growth on routine Mac Conkey medium (lactose non-fermenting pale colonies) and on Brain Heart Infusion agar (greenish pigmented colonies) which were oxidase test positive & all confirmatory test positive for Pseudomonas aeruginosa. Antimicrobial susceptibility of all the isolates were performed by the Modified-Kirby Bauer disc diffusion method with Ceftazidime, Piperacillin, Piperacillin+Tazobactam, Imepenam, Lomefloxacillin, Tobramycin, Polymyxin B, Ofloxacin, Ticarcilin+Calvulanic acid & Amikacin according to CLSI guidelines.
Results: In this study, maximum isolates of Pseudomonas aeruginosa isolated from various samples were sensitive to Imepenem (90%), Ticarcillin+Clavulanic acid (79%) followed by Piperacillin+Tazobactum (73%) & polymyxin B (73%), Piperacillin(64%),Amikacin(52%) Ceftazidime (48%), Tobramycin (48%), Ofloxacin (47%), Lomefloxacillin (46%).
Conclusion: Since Imepenem showed highest sensitivity, clinician should prefer Imepenem in case of Pseudomonas aeruginosa infection. In case of carbepenem resistance combination of drug like Ticarcillin+Calvulanic acid, Piperacillin+ Tazobactum should be used as an alternative. Key Words: Pseudomonas aeruginosa, Antimicrobial susceptibility,