Antimicrobial activity of piperine based zinc oxide nanoparticle against dental pathogens
Author(s): Kritheka CK* and Sarita Bandari
Abstract
Background: The Piperaceae family, including black pepper, is known for its antibacterial properties. The oral cavity is a major source of microorganisms, including plaque biofilms, which cause infections. Advancements in nanotechnology have led to a growing interest in antibacterial therapies based on nanoparticles. Black pepper, a member of the Piperaceae family, contains polyphenolic substances used in traditional medicine for treating illnesses like melano derma and leprosy. In this study, Piperine incorporated with zinc oxide nanoparticles was tested for efficacy against dental pathogens. Aim: To determine the antimicrobial activity of Piperine based zinc oxide nanoparticle against dental pathogens Materials and methods: Zinc acetate dehydrate and NaOH were used as precursors in the direct precipitation technique to create zinc oxide nanoparticles, which were then examined under a scanning electron microscope. Using the 2, 2-diphenylpicrylhydrazyl (DPPH) experiment, antioxidants' capacity to scavenge radicals is assessed. 2.2’-azino-bis (3-ethylbenzothiazoline-6-sulfonate) are the ABTS test used to quantify the interaction between an antioxidant and the cation of the pre-generated ABTS+ radical. The Zone of Inhibition test is used to assess a pathogen's sensitivity to antibacterial agents or resistance to them. The outcomes were assessed. Results and discussion: In this study zinc oxide nanoparticles were doped with piperine and SEM was demonstrated. The SEM images revealed that the uniform distribution and almost spherically shaped piperine mediated ZnO NPs is observed. The DPPH assay & ABTS assay shows antioxidant activity of ascorbic acid, piperine & piperine doped ZnO NP against oral pathogens. In DPPH assay & ABTS assay, there was an increased inhibition rate in piperine mediated ZnO NP when compared ascorbic acid and piperine. Therefore results concluded that piperine mediated zinc oxide nanoparticles showed very good antimicrobial activity against all oral pathogens such as S. mutans, C. albicans and E. faecalis. Conclusion: Nano-dentistry is a developing field with the potential to address new and improved applications in dentistry, and it has provided a new avenue for revolution in oral care. Our study conclusions suggested that PP-mediated ZnO NPs may provide a useful nanomaterial for the creation of drugs intended to treat oral infections. ZnO nanoparticles mediated by PP showed remarkably potent antibacterial activity against S. mutans, Candida albicans, and E. faecalis, among other oral pathogens. When using 28-nm PP ZnO NPs against E. faecalis, a greatest inhibition zone was seen.