Antimicrobial Assay of Novel Zirconia and Silver Phyto Nanoparticles Biosynthesized using Ocimum Sanctum and Syzygium Aromaticum Extract-A Preliminary Study
Introduction: Dental caries is caused by acidogenic bacteria and pathogens present in oral cavity. In orthodontics, white spot lesions or incipient caries is an iatrogenic side effect that occurs due to the fixed appliances placed in oral cavity, promoting niche for acidogenic bacteria. New vistas of caries management involve introduction of nanotechnology in toothpastes, bonding agents, varnishes, remineralizing agents, mouth rinses etc. Through this preliminary study we demonstrate the antimicrobial activity of novel silver and zirconia nanoparticle synthesized using Ocimum sanctum and Syzygium aromaticum extract against Streptococcus mutans, Lactobacillus and Candida albicans.
Materials and Methods: Using extracts of Ocimum sanctum and Syzygium aromaticum, silver and zirconia nanoparticles were synthesized. Formation of Silver and Zirconia nanoparticles was confirmed by surface plasmon spectra using UV- Vis spectrophotometer. The morphology of the crystalline phase of nanoparticles was determined from transmission electron microscopy. The average sizes of the Silver and zirconia nanoparticles was in the range of 20-25nm and 85nm, respectively. The antimicrobial activity was assessed against Streptococcus mutans, Lactobacillus and Candida albicans. Results: Silver and zirconia nanoparticles exhibited good antimicrobial activity against the pathogens. The antimicrobial activity of Silver nanoparticle was more pronounced than zirconia nanoparticle.
Conclusion: silver and zirconia nanoparticles synthesized using Ocimum sanctum and Syzygium demonstrated good antimicrobial activity. The plant-based nanoparticle synthesis is a fast, novel and eco-friendly method and the nanoparticles generated by this method shows excellent antimicrobial activity against streptococcus mutans, lactobacillus and candida albicans.