Assessment of Gingival Crevicular Fluid Levels of "Vitamin-D Binding Protein" in Periodontitis Patients with Stage II-IV
Background: Vitamin D Binding Protein (DBP) is the major transporter protein of vitamin D in the bloodstream. In addition, it plays an essential role in immunomodulatory and anti-inflammatory functions. There are limited studies regarding the relationship of this protein with periodontitis, which measured its level in saliva, serum, and gingival crevicular fluid and found a link between periodontal health and periodontist with the level of DBP.
Aims: To assess DBP level in the gingival crevicular fluid of periodontitis patients with (stages II, III, and IV) compared to healthy controls, also to measure clinical parameters and correlate them with the level of DBP in periodontitis patients. Materials and methods: A total of 49 subjects participated in the study; the Probing Pocket Depth (PPD) and the Clinical Attachment Level (CAL) were measured clinically. Gingival crevicular fluid was collected from each individual. An Enzyme- Linked Immunosorbent Assay (ELISA) was utilized to detect DBP levels.
Results: The level of DBP was found to be decreased in periodontitis groups compared with a healthy control group (p-value <0.001). A significant difference was reported between the healthy control groups and stage III and stage IV. No significant correlation was found between periodontal parameters and DBP level in the gingival crevicular fluid.
Conclusion: According to this study, reduced DBP level was noticed in the gingival crevicular fluid of periodontitis patients in comparison with healthy controls. This suggests that DBP is involved in both periodontal health and the pathogenesis of periodontitis.