Journal of Research in Medical and Dental Science
eISSN No. 2347-2367 pISSN No. 2347-2545

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Comparative Study of Modified Chlorhexidine Toxicity, Chlorhexidine and Sodium Hypochlorite on Gingival Fibroblast Cells L929 In Vitro

Author(s): Samira Shahsiah, Eskandar Moghimipour, Ali Khodadadi, Maryam Sharifi

Abstract

One obvious and good properties of sodium hypochlorite is capacity of tissue solubility which has effective role in direct root canal cleaning, necrotic tissues and root canal system complexity. Chlorhexidine, in spite of all advantages that have than sodium hypochlorite, lacks this property. Superficial active factors or surfactants are materials that are used extensively as irritant, emulsifier, disinfection and solution additive. Surfactants are different types including benzalkonium chloride 4% and sodium lauryl Sulfate 2%. In this research, sodium dodecyl sulfate was used. Gingival fibroblast cells are kept in 25 cm2 flasks in 370C temperature. And then they are cultivated in Gibco Dulbecco’s Modified Eagle Medium (DMEM). 96 wells plates with 50 microliter are filled with growth medium with 2000 fibroblast and it is placed in incubator for 24 hours. After 24 hours, growth medium is thrown away and all plates are washed with phosphate buffered saline (PBS). All experimental steps are performed for preventing samples being contaminated under hood. The experiment was carried out as triplicate (3 wells cell). Results: In order to perform a research, Tukey-test and Duncan test were applied for explaining research variable. Results: toxicity rate of under tested solutions on fibroblast cells for group 1-4 are respectively (right to left) 13.75, 51.75, 20.75 and 51.75. Results of comparing means in four groups in landa level 4 indicated that groups are not significantly different in toxicity rate. results of this research indicated that this mixture can be used as an appropriate candidate for replacing sodium hypochlorite based on its disadvantages which needs to be studied more.

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