Comparison of Antibacterial Activity of Three Endodontic Sealers against Enterococcus faecalis
Author(s): Salma omidi, Narjes Hoshyari, Ali Reza Mirzadeh, Mehran Ebrahimzadeh Hassanabadi, Mohammad Ahajan, Jamshid Yazdani Charati,Azam Haddadi Kohsar
Abstract
one of the important property of endodontic sealer is antibacterial activity. This study aimed to compare the antibacterial properties of three common and widely used sealers: MTA-FillApex, AH Plus, and AH26 against Enterococcus faecalis. In this study, 25 Blood Agar plates were prepared, in each of which 5 wells were devised. In three wells, three sealers of MTA-FillApex, AH Plus, and AH26 were placed. Ampicillin was poured into the fourth well as the positive control group. Finally, distilled water was poured into the fifth well as the negative control group. Concurrently, 1/10 ml of Enterococcus faecalis bacteria suspension with a concentration of 0.5 McFarland was cultivated on the plates. Following 48 hours of incubation, the diameter of inhibition zone around the wells was measured. Finally, the obtained results were analyzed by one-way ANOVA, fisher LSD, Fisher individual 95% Cl. The results of our study on 25 plates after 48 h indicated AH26 sealer with the mean zone of inhibition diameter of 16.44 mm had the greatest antibacterial effects. AH26 was followed by MTAFillApex with the mean zone of inhibition diameter of 15.44 mm. Finally, the weakest antibacterial effect was related to AH Plus sealer, with the mean zone of inhibition diameter of 10.2 (p<0.1000). According to our study, AH26 sealer has the greatest power and antibacterial properties against Enterococcus faecalis bacteria, followed by MTA-FillApex, and AH plus (as the weakest antibacterial agent against the mentioned bacteria).
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