Comparison of the Bactericidal Effects of Two Different Diode Laser Wavelengths 810 NM And 980 NM within the Treatment of E. Faecalis-Infected Root Canals
Background: A variety of causes can lead to endodontic treatment failure, including bacterial persistence, insufficient root canal cleansing or obturation, incorrect coronal seal, and untreated canals (missed canals). The endodontic treatment failure arises from many reasons and the main one is the existence of some bacterial species inside the root canals like the Enterococcus faecalis , which is the most common type. It possesses unique characteristics that enable it to evade disinfection leading to radicular inflammation. Newer laser technology disinfection techniques have been recommended to be effective for routine endodontic treatment as a result of the development of the potent antimicrobial capabilities of lasers in recent years.
Aim: In vitro study, comparative between the influences of two various wavelengths of diode laser (810 nm and 980 nm) within the root canal system against Enterococcus faecalis during endodontic treatment.
Materials and methods: Preparation and sterilization of a total of forty canals before being contaminated with Enterococcus faecalis bacteria and cultured for two weeks. After that, the human permanent teeth were separated into four groups at random. A (control group) specimens that have not been treated, group B (its specimens were treated with 17% EDTA and sodium hypochlorite at 5.25%), group C (specimens radiated with 810 nm diode laser), and Group D (specimen radiated with 980 nm diode laser). After the disinfection steps, specimens were plated on blood agar media in order to count the number of colonies for experimental groups.
Results: In both experimental groups, laser irradiation reduced the number of bacterial colonies. The reduction in the microbial count was significantly greater in the 810 nm laser group (70.8%) compared to the 980 nm laser group (29.1%). Using Dunnett's T3 test, which demonstrated significant differences among the groups with an exception of that between-group C and other groups which was not a significant difference. However, the greatest bacterial eradication was achieved when sodium hypochlorite was used in conjunction with the EDTA treatment (81.6% CFU/ml reduction).
Conclusion: The 810 nm diode lasers sterilized and killed E. Faecalis bacteria more successfully than the 980 nm diode laser. However, when sodium hypochlorite at 5.25% was utilized in combination with the 17% EDTA treatment, the most bacterial clearance was observed.