Determining the Sex of the Fetus through the Serum of Pregnant Mothers at 8-12 Weeks Was Determined by the 4 STR Markers (DYS393, DYS460, DYS388, DYS391) Specific Chromosomes Y
Author(s): Niloofar Haji Kermani, Ali Nazemi, Vida Shafti
Abstract
Non-invasive sex-determination methods, such as the use of ultrasound waves, are performed in the second trimester of pregnancy, which is relatively late in women who are at risk for X-related illness (sex), But today by creating new non-invasive methods in the second month, one can identify the sex of the fetus. Therefore, in the present study, the aim of this study was to find a non-invasive method for determining the sex of the fetus through cff DNA based on the STR marker at the Y chromosome level. This cross-sectional study was performed in the second six months of 1396. For this purpose, 23 blood serum samples were collected from the mother and DNA extraction was performed using DNA extraction kit. Primers were extracted from the paper(Butler, etal. 2002). Then real time reaction was performed on the DYS393 DYS396 DYS396, DYS388, DYS39 genes and finally the data were analyzed by SPSS software using chi square and ANOVA. The results in this study indicated that marker 388 was more specific than other markers with 73.9% then the marker 460 with 69.6% and the marker 391 with 69.6% and the marker 393 with 52.6%. Therefore, the specificity of marker 388 is better than other markers for gender determination it is also suggested that this study should be done with a higher statistical population in order to obtain more accurate results and use STR markers for further examination so that information can be obtained in the future.
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