Distribution of Human Papilloma Virus-16 E7 oncoprotein in Iranian Patients with Cervix Cancer
Author(s): Ahmad Piroozmand, Fateme Jafariazad, Mohammad Niakan, Azad Khaledi, Hale Piroozmand
Cervical cancer is a common neoplasm of the female genital tract. Persistent infection with high-risk human papillomavirus (HPV), particularly HPV 16 is associated with cervical carcinoma due to the ability of producing the viral Oncoproteins (E6 and E7) can abrogate the cell cycle. This study aimed to determine the distribution of human papilloma virus-16 E7oncoprotein in Iranian patients with cervix cancer.Of the 150 paraffin blocks from patients with pre-cancerous lesions and cervical cancer of Tehran hospitals, Iran, 69 samples were confirmed by the pathologists as cervical cancer. Then, their DNAextracted using the phenol/chloroform method and to evaluate the HPV16 E7, primer related to E7 gene was used.Then, the data were analyzed using Chi-square test (Fisher exact test). The mean age of patients was 50±2.3 years. Most of the patients were from the age group of 41-51 years and all were married. Among total numbers of patients, 53.6% were positive for HPV16. The mostwomenwithcancer belonged to age-group 41-50, OCP consumer and women who got married under 18 years old.Results of the present study showedthat the HPV16 is one of the major genotypes that are associated with cervical cancer in Iranian patients which should be considered in prevention and vaccination programs. In addition, screening of a highly risky human papillomavirus, especially type 16 can be recommended for all patients suspected with cervical cancer using PCR of E7 that is accurate and effective.