Effect of Different Storage Solutions and Autoclaving on Shear Bond Strength of Composite to Dentin
Author(s): Neda Jarahi, Alireza Borouziniat*, Lida Jarahi, Amir Hossein Nejat
Purpose: Extracted teeth contain various types of micro-organisms which possess the risk of cross infection also the procedure of disinfection can be harmful to the structure of in-vitro tooth samples. To investigate the effect of storage solutions and autoclaving on the shear bond strength of composite resins to dentin.
Materials and Methods: Sixty human molar teeth were randomly allocated into different groups according to storage solution, and autoclaving: (G1) fresh teeth, (G2) fresh teeth autoclaved at 121°C, 15 psi pressure; (G3) storage in Choloramin-6 months; (G4) G3+autoclave; (G5) storage in Thymol-6 months; (G6) G5+autoclave. The shear bond strength of composite to dentin was assessed by Instron machine. Kruskal-Wallis and Mann-Whitney test were performed using SPSS software (version 11.5) for analysis.
Results: The results showed that the storage of teeth in Chloramine solution significantly increase the bond strength (Pvalue< 0.05). However, storing in Thymol had no significant effect on bond strength (P-value>0.05). Autoclaving process did not significantly affect the shear bond strength in comparison to control and even after solution storage (Thymol/ Chloramine) (P-value>0.05).
Conclusion: For more than 6-month storage Thymol is a better solution to store the teeth in comparison to Chloramine. Autoclaving recommended before in-vitro studies for sterilizing extracted teeth.
Key message: 6 months storing teeth in Thymol had no significant effect on bond strength of composite to dentin. According to the results, Thymol is a better solution to store the teeth in comparison to Chloramin. Autoclaving also did not affect the bond strength significantly.