Effects of Ozonated Water on Micro Leakage between Enamel and Fissure Sealants Prepared by Different Etching Technique (An in vitro Study)
Author(s): Baraa M Jabar*, Muna S Khalaf
Aims: the aims of this (in vitro study) were to investigate the effect of Ozonated Water pre-treatment on the micro leakage
of fissure sealants prepared by different surface etching modalities.
Material and methods: According to the pre-treatment approach, 80 standardized premolar teeth were separated into five
groups (n=16). Group 1: Universal Bond Quick (self-etching)+ozone application+Fissure Sealant (FS). Group 2: Universal
Bond (self-etching)+FS (without ozone). Group 3: Universal Bond (etch-and-rinse)+ozone application+FS, Group 4:
Universal Bond (etch-and-rinse)+FS (without ozone), Group 5: phosphoric acid etching+FS (control).
All teeth's fissures were sealed with fissure sealant material after surface pre-treatments. The samples were then subjected
to thermos cycling (1 minute of 500 cycles of 5°C/55°C for each sample). All surfaces of the teeth were covered twice with
nail polish up leaving 1 mm away from the fissure sealant. For 24 hours, all of the teeth were soaked in a 3 percent
methylene blue dye. The teeth were sectioned into three parts Bucco-lingually by SAW to investigate micro leakage. A
stereomicroscope was used to photograph the item. The extent of dye penetration was measured in millimetres using image
Results: The findings showed that micro leakage was lower in group with using Ozone pre-treatment than when not using
Ozone pre-treatment either in self or in total etching groups with significant difference in each group.
Conclusions: Ozone pre-treatment positively affected the fissure sealants marginal integrity.