Evaluation of In-Vitro Anticancer Activity from Rhizophora Mucronata Mangrove Leaf Extracts against Lung Cancer Cell Line
Author(s): Aksha Sharen, P Sivaperumal, Lakshmi T* and Anitha Roy
Abstract
Introduction: Herbal medicines play an important role in cancer prevention. Mangroves have been used worldwide due to the presence of various bioactive metabolites in it. The specific medicinal properties of Rhizophora mucronata have been an interest in preventing breast cancer. Mangroves Rhizophora mucronata belongs to the family Rhizophoraceae. The leaf extract reveals various roles in folk remedies to treat various diseases. The aim of the study is to determine anticancer activity of Rhizophora mucronata mangroves leaf extract against lung cancer cell line Methodology: The Rhizophora mucronata mangrove leaf samples were collected. The collected sample was washed and then dried for and turned into fine powder. A 25g dried powdered Rhizophora mucronata sample was mixed with 100ml of Ethanol and allowed to place for 24 hours at ambient temperature. Then the mixture was passed through Whatman filter paper then the filtrate was centrifuged at 3000rpm for 10min and further filtered by 0.45μm syringe micro filter. At last, the solvents are evaporated via vacuum rotary evaporator until samples obtain in powder form. Then the sample was stored in a shadowy aluminium container at 4°C for further analysis. The proliferation of MCF-7 cells was assessed by MTT assay. Results: The present study demonstrated the anticancer activity of Rhizophora mucronata mangrove leaf extract against lung cancer cell line and the result is graphically represented. Conclusion: The obtained result established the anticancer activity of Rhizophora mucronata mangrove leaf extract against lung cancer cell lines.