Evaluation of the Antibacterial Efficacy of Electrolyzed Oxidizing Water as an Irrigant against Enterococcus faecalis (An In vitro Study)
Author(s): Noor A Khait* and Muna Saleem Kalaf
Abstract
Background: Disinfection of the pulp space by extirpation of the infected pulp, microorganisms and their toxins all are essential basis for successful endodontic treatment.
Objective: This study aimed to investigate the antibacterial effect of electrolyzed oxidizing water and sodium hypochlorite in lowering bacterial infectious disease of the root canal.
Materials and Methods: 45 single rooted permanent human teeth were properly cleaned, shaped, and disinfected. All of the teeth samples were contaminated with Enterococcus faecalis for two weeks at 37°C. After that, the teeth were categorized into 3 groups. 15 for each of 3% sodium hypochlorite, 15 for electrolyzed oxidizing water, and 15 for normal saline as a control group. Pre-and post-irrigation samples were collected using paper points. After 24 hours, the bacterial growth was assessed. The number of bacteria colonies was then counted. The data was evaluated with SPSS and tested utilizing the One-Way ANOVA, Shapiro-Wilk test, and Dunnett's T3 posthoc test, with a significance level of 0.05.
Results: Antibacterial effectiveness: Both sodium hypochlorite and Electrolyzed oxidizing water displayed better effectiveness when compared to normal saline (P<0.05), comparatively, 3% sodium hypochlorite demonstrated the greatest efficacy against E. faecalis biofilm. Electrolyzed oxidizing water than normal saline, percentage of antibacterial effectiveness was 97. 923%, 97.018% and 31.614 respectively with significant difference between them.
Conclusions: Electrolyzed oxidizing water had comparable effectiveness against biofilm of E. faecalis to sodium hypochlorite when it was used as an endodontic irrigants solution.
Keywords: Sodium hypochlorite, E. faecalis, Electrolyzed oxidizing water, Electrolysis