Evaluation of the Wound Healing Potency of Myrtus communis Aqueous Extract on Diabetic Rat
Author(s): Ghasem Habibi, Taha Fereydouni, Mahmood Amini*, Hossein Sarmadian, Manijeh Kahbazi*, Siamak Rakei, Mohammad Arjomandzadegan
Background: Diabetic patients have a main problem on treatment and management of diabetic wounds. Wound healing is accelerated by herbal material. Aim of the study was to investigate the wound healing potency of Myrtus extract in diabetic rat.
Methods and Materials: In this experimental study, 20 Wistar male rats were randomly divided into 2 groups as the control and the treatment by M. communis extract. Two days after streptozotocin injection all rats were tested for blood sugar and were kept for 4 weeks. A full-thickness circular excision was inflicted approximately 1 inch of diameter of the predetermined area on the dorsal side of each rat using a sterile surgical blade. Treatment group treated 3 times a day for 13 days with aqueous extract of M. communis. The control group was left untreated. The extract concentration was 200 ppm per litre and was made by a simple distillation system. The wound area was calculated on days 3,7,11,13. Finally, assessment of the cutaneous wound healing between the groups was carried out by histopathologic data and statistical tests.
Results: Fasting blood sugar in diabetic rats was 300 mg/dl ± 30 after 48 hours and remained for four weeks. In control group, infection, secretion and inflammation were proved in wounds from 2nd day after inoculation, but without such evidences in test group. Histopathological evaluation indicated re-epithelialization, healing and reduced the inflammation on day 13 in comparison to control (P<0.001). Area changes and wound healing in the test group treated with the extract was significantly better than control group (P<0.001).
Conclusions: Our results proved that Myrtus Communis aqueous extract promoted wound healing and was controlled infection and proposes as a therapeutic material for wound treatment in clinical trial investigations.