Green Synthesis of Selenium Nanoparticles from Banana Stem Extracts and their Cytotoxic Effect
Background: Nanoparticles are made using a variety of traditional processes. To maintain stability, hazardous compounds are required as capping agents, resulting in environmental toxicity. As a result, we must switch to "green synthesis." As a result, the purpose of this research was to determine the cytotoxicity of selenium nanoparticles supplemented with banana stem cell extract. The banana was chosen for this investigation because of its inherent anticytotoxic characteristics. Aim: Aim of the study was green preparation of selenium nanoparticles from banana stem and to check its cytotoxicity evaluation. Material and methods: Cytotoxic effect of prepared selenium nanoparticles from banana stem was assessed using Brine Shrimp Assay respectively at 5 µL, 10 µL, 20 µL, 40 µL, 80 µL. Results: The selenium nanoparticle is safe to be used in the dental materials till 20 µL concentration. Although at 40 µL, 80 µL concentrations cytotoxicity was observed. Conclusion: Within the limits of the study it can be concluded that selenium nanoparticles can be safely used in concentrations upto 20 µL as periodontal dressing or incorporated as bone grafts in periodontal regeneration.