Histomorphometric Evaluation of the Effect of Local Application of Moringa Oliefera/Marine Collagen on Bone Healing in Rats
Backgrounds: Bone repair is a complex multistep process, flavonoids group present in Moringa Oliefera and Marine collagen helps to increase the bone minerals contents density of bone.
Objectives: the aim of study was to evaluation the differences between the healing process when used of Moringa Oleifera (MO), Marine collagen (MC) and their combination (MM) on bone defects (experimental groups) and bone defect that heal normally (CONT) (control group).
Material and Methods: 20 albino rats were anesthetized and both femurs prepared by drilling intrabony defects (2mm diameter, 3mm depth). In 10 rats, right defects consider control while left defects treated with MO, in other 10 rats right defect treated with MC while left defects treated with MM. scarification done in 2 periods: 2 and 4 weeks. Histological and Histomorphometric analysis of bone microarchitectures. Bone cells were counted. Also, trabecular number, trabecular area and bone marrow area per mm2 were measured using image j program.
Results: Histological result revealed acceleration of bone healing in combination group (MO&MC) than that other groups. Highly significant differences were seen between combination of MO with MC and control group in both duration for almost all histomorphometric parameters used in this study.
Conclusions: Combination of MO with MC improve bone healing and increase osteogenic capacity.