Improved Antimicrobial Efficiency of Aqueous Crude Extracts of Green Tea against Oral Microbiota
As most of the plants have biologically active constituents, our present study focuses on the antimicrobial activity of aqueous crude extracts of green tea and black tea under aerobic and anaerobic incubation conditions using old extractive technique, maceration method by using both hot water and cold water and are subjected to evaporation to get semisolid consistency mass which are then air dried to get the powdery mass. Thus, obtained powders are stored in well closed containers and are made into solutions of desired concentrations of 25, 50, 75 and 100mg/mL to observe their antimicrobial activities against oral microbes under both aerobic and anaerobic incubation conditions thus inferring their antimicrobial activity against both aerobic and anaerobic oral microbes. The antimicrobial activity is determined by zones of inhibition shown by the extract dilutions which is compared to the zones of inhibition shown by commercial antibiotics used in oral cavity like tetracycline, gentamicin and amoxicillin. The cold water crude extract of green tea is found to have significant antimicrobial activity. The most prominent zone of inhibition is shown by the crude extract (cold water) combination of green tea and black tea in the ration of 1:1 at concentration of 75mg/mL and the zone of inhibition is found to be 31mm. Thus, inferring the extracts have significant antimicrobial activity against oral microbes and hence this investigation reveals the scope of formulating and developing dosage forms that can be used to treat oral infections.