In Vitro Assessment of Microbial Contamination and the Disin | 82375

Journal of Research in Medical and Dental Science
eISSN No. 2347-2367 pISSN No. 2347-2545

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In Vitro Assessment of Microbial Contamination and the Disinfecting Adequacy of Alcohol Solution on Orthodontic Pliers

Author(s): Batool H Al-Ghurabi*, Maha F Almelan and Zainab A Aldhaher


Background: The oral cavity contains several types of microorganisms that frame a complex environment and a different and regularly pathogenic microbiota. Subsequently, uncommon consideration ought to be paid to contamination control and biosafety in dentistry. Objectives: The point of the show think about was to assess the microbial defilement of orthodontic pliers and disinfection efficacy of ethanol alcohol. Materials and Methods: Ten sterilized pliers were enrolled in this study. A sterile cotton swab was rolled over the internal surfaces of the pliers' beaks after clinical use and after disinfecting with ethanol alcohol solution. Then the samples were transported immediately for culture and identification of microorganisms. Results: Microbial contamination was detected on all pliers after used. Streptococcus spp. was found to have the highest percentage, while Escherichia coli and Bacillus spp. scored the lowest percentage of the isolated microbial strains. Upon disinfection with 70% ethanol alcohol solution revealed reduction in number of colonies of different microorganisms. Conclusion: These finding suggested that like any other dental tools orthodontic pliers were contaminated after use in clinical cases, so they must be sterilized after each use in patients. In addition, this study indicated that the disinfection with 70% ethanol alcohol is the not efficient method.

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