In vitro Bioactivities of Aqua Alcoholic Extracts of Plant Boerhavia Diffusa Linn.
Background: Herbal extract of various plants have been reported to have cytotoxic and antioxidant effects. However, nanoparticle synthesis from these plants requires high resources and technical expertise. There is a need for a cost effective Nano formulation preparation method. Boerhavia diffusa is an herbal plant used for its cytotoxic and antioxidant effects in various diseases. The objective of the present study is to evaluate the in vitro antioxidant and cytotoxic activity from the aqua alcoholic extracts of plant Boerhavia diffusa. Materials and Methods: The current study was a cross-sectional observational analytic study. Our study includes 150 children having the age group of 3 to 12 years who visit the Department of Pediatric Dentistry in the College of Dentistry, King Khalid University. One hundred and fifty children (range 3-12 years) were included whose parents reported snoring on a regular basis and signs or symptoms of OSA. A self-administered questionnaire was prepared in both Arabic and English languages and distributed to all the participants. All the data were collected and analyzed by using SPSS version 21. Materials and Methods: Assessment of antioxidant activity is carried out by in vitro method Diphenyl-1-picryl hydrazyl radical (DPPH) scavenging method and cytotoxic activity live brine shrimp assay by counting the number of live brine shrimps after 24 hours and 48 hours. Values are tabulated and spearman correlation analysis was done in SPSS software version 23. Results: The activity of the aqua alcoholic extract was compared with standard ascorbic acid by measuring absorption intensity in the spectrophotometer at the wavelength of 517 nm. While increasing the concentration of plant extracts the percentage of DPPH inhibition also increased which showed positive correlation(r=1). As a result of the death of numerous nauplii, cytotoxic activity increased, which was measured after 48 hours and it shows negative correlation(r=-1). Conclusion: Boerhavia diffusa have cytotoxic and antioxidant properties. The natural plant based nanoparticles with its phytoconstituents in a single pace adds up a huge advantage in eco-friendly nano research.