Investigating the Susceptibility and Death Kinetic of Pseudomonas Aeruginosa Bacterium Standard and Clinical Strains to the Copper Oxide Nanoparticle
Author(s): Sajjad Babaei, Ardeshir Abbasi, Omid Mansouri zaveleh, Zohreh Amiri, Nasrollah Sohrabi
Abstract
Microbial resistance is considered as one of the challenges in coping with infectious diseases. Thus, to find or synthesize new antimicrobial substances is very important. Copper oxide (CuO) has drawn the attention of the researchers due to its antibacterial effect in coping with microbial resistance. In this research, the antibacterial effects of copper oxide nanoparticles on the standard and clinical strains of Pseudomonas aeruginosa, which its new strains have been associated with microbial resistance, were examined and compared. In this experimental-interventional research, a copper oxide nanoparticle was synthesized at a size of 33 nm using copper sulfate chemical reduction method. Then, the antibacterial effects of copper oxide nanoparticle on standard strain (ATCC 27853) and clinical strain of Pseudomonas aeruginosa were examined using minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and bacteria death kinetics. Data derived from investigating the bacteria death kinetics were analyzed using statistical tests at a significant level of p value (<0.05). Results: The MIC obtained in the standard strain and clinical strain with 33 nm nanoparticle was 5000 mg / ml and 2500 mg / ml, respectively. In addition, the obtained MBC in the bacterial standard strain and clinical strain with 33 nm nanoparticle was ≥ 10,000 IU / ml and ≥5000 IU / ml, respectively. The effect of nanoparticle on each of the strains was at significant level compared to control group. However, the difference between the effects of nanoparticle on strains in comparison to each other was not at the significant level. The current research revealed that the copper oxide nanoparticle had an Impressive Inhibitory effect on the standard and clinical strains of Pseudomonas aeruginosa, depending on nanoparticle’s concentration and size. Thus, studies conducted in this area provide appropriate conditions to achieve new antibacterial substances.
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