Investigation into the Experimental Effects of Juglans regia Cyclohexan Extract on Arginase Gene Expression (ARG2) in Adult Male Diabetic Rats with STZ and Healthy Rats
Author(s): Karim Kheradmand, Mehrdad Shariati, Gholamali Jelodar, Mokhtar Mokhtari, Saeed KhatamSaz
Background and Aim: Diabetes type 2 is a disease with functional impairment in body. The use of medicinal herbs in the treatment of diabetes is traditional in ancient medicine. Antioxidant compounds of walnut leaf are effective in reconstructing pancreatic beta cells. This research was conducted to study the effects of walnut leaf Cyclohexanin extract on the expression of arginase gene (ARG2) in normal rats and diabetic rats.
Methodology: 50 Sprague dawley rats (200 g-250 g) were selected and divided into 10 groups. A group of rats did not receive any extracts of walnut leaf. Two normal control groups and two diabetic groups (with STZ) were gavaged with Cyclohexanin extract of walnut leaf for 6 weeks. At the end of the course, the primers of this research were designed. Real Time PCR (SYBR Green) was then used to extract and check the gene expression. To analyze Real Time PCR data, Fold change was first calculated and then the data were analyzed with REST software (ver-9).
Results: The results of the analysis show that the Arginase gene is evenly expressed in both groups of CN and CT-C and. But in the DT-C treatment groups, the Arginase gene is significantly reduced compared to the CD group. According to the P-value, which is 00/0 and the rate of Fold Change, which is 0/027, the results of the analysis showed that the gene expression in the diabetic group treated with the cyclohexan extract (DT-C)reduces significantly compared the diabetic control group (CD) and this deference is significant (P<0.05)
Conclusion: Walnut leaf has unsaturated fatty acids. These acid compounds probably inhibit the activation of the arginase enzyme gene by their effects. The inhibitory action of the gene expression by Cyclohexan extract of walnut leaf can be likely attributed to Gallic acid and Caffeoylquinic acid in the walnut leaf, which prevents gene transcription and thus turns off the gene.