Ion-Pair Complex of Ketotifen Fumarate with New Reagent and its Extraction by LLE and DLLME
Author(s): Araf I Jabbar*, Ali I Khaleel and Mohammed Z Thani
Abstract
A novel, simple, fast, and reproducible liquid-liquid extraction and dispersive liquid-liquid micro extraction (DLLME) combined with UV-visible spectrophotometric methods were developed utilizing Sodium (E)-4-chloro-5-((2-hydroxynaphthalen-1-yl) diazenyl)-2-methylbenzenesulfonate (SCMS) as a reagent for the estimation of ketotifen fumarate (KTF) in a pure state and in the pharmaceutical dosage form. The suggested methods are based on the formation of the red ion-pair complex of ketotifen fumarate (KTF) with SCMS reagent in britton pH=2 buffer. The extracted red complexes showed absorbance maxima at 490 nm. Beer?s law is obeyed in the concentration ranges (5.0-50.0 mg/L) for (LLE) and (1.0-10.0 mg/L) for (DLLME), respectively. The molar absorptivities 1.36×103 and 15.8×103 L.mol-1.cm-1 for LLE and DLLME procedures, respectively, as well as the Sandell`s sensitivity, 0.31 and 0.26 µg.cm-2 for both methods, which indicated the high sensitivity of the suggested methods. The percent relative standard deviation (RSD%) 1.8-5.1% and 0.5-4.6 % for LLE and DLLME refer to the high precision of the proposed method.