Measurement of doxycycline in pharmaceutical Samples with SnO2 nanoparticles by spectrophotometric method
Author(s): S.Batool hosseini, Mahmoud Ebrahimi, Mohammad Reza Bozorgmehr
In this work, The SnO2 nanoparticles were characterized which developed a novel SnO2 nanoparticleassisted matrix solid-phase extraction (SnO2 -SPE) method for extraction of doxycycline from pharmaceutical and environmental samples. A simple, rapid and sensitive method termed as solid phase extraction (SPE) combined with spectrophotometric method has been proposed for the determination of trace amounts of doxycycline (DOX) in pharmaceutical samples. The influences of effective parameters on the extraction efficiency were studied. The effect of the different parameters such as pH, extraction time, effect of the salt, nanoparticle amount and extraction temperature has been studied. Under optimized condition, the method was successfully applied to the extraction of DOX from aqueous samples and relative recovery amount of 89%, detection limit of 25μg L-1 and a relative standard deviation (RSD) of 2.3% were obtained. The method linear response was over a range of 10–50000μgL−1 with R2 = 0.991. The whole procedure showed to be conveniently fast and high extraction efficiency of DOX from real samples.