Methodology for Determining Sensitivity of Microorganisms to Sorption Drugs
Author(s): Vladimir Buhanov, Alexander Vezentzev, Spichak Irina, Ulyana Krut*, Tatyana Shevchenko, Larisa Kadutskaya and Elena Arseenko
Abstract
This study was undertaken to design methodological recommendations for determination of sensitivity of microorganisms to sorption drugs. The basic premise of it was long-term experiments that give optimal solutions for the studied issue. To our knowledge, no research has been carried out on the methodology for determination of sensitivity of microorganisms to sorption drugs. Realizing this gap, it is needed to create a modern clinically reasonable development based on the existing methodological instructive regulations 4.2.1890-04 “Determination of the sensitivity of microorganisms to antibacterial drugs”. The study is focus on the most significant statements of determining the sensitivity of in vitro microorganisms to sorption drugs by the method of serial dilutions in a liquid nutrient medium. We have discussed the questions about interpretation of the results of determining the sensitivity of microorganisms to sorption drugs from a clinical and microbiological point of view. Our finding reveal that the developed methodological recommendations systematize the modern approaches of determination of the sensitivity of bacterial pathogens to sorption drugs, taking into account the recommendations of the European Committee for the Determination of Sensitivity to Antibiotics, as well as the US National Committee for Clinical Laboratory Standards.