Phyto-assisted Synthesis and Assessment of Anti-Inflammatory and Antioxidant Properties of Copper and Graphene Oxide Nano Composite Reinforced with Amla Extract-An In-Vitro Study
Author(s): Gaurav N Ketkar, Sankari Malaiappan* and Rajeshkumar S
Abstract
Background: conventional methods used for synthesis of nanoparticles use toxic chemicals, thus leading to toxicity in the environment. Hence, we need to shift to “green synthesis”. Previously done have shown excellent antimicrobial properties of the copper and graphene oxide nanocomposite reinforced with amla extract. Hence, this study was conducted to assess the anti-inflammatory and antioxidant properties of copper and graphene oxide nanocomposite reinforced with amla extract. Copper causes cell wall lysis by leakage of intracellular substances hence is an excellent antimicrobial agent, graphene oxide for its barrier and structural strength hence these were chosen to create a nanocomposite. Aim: Aim of the study was phyto-assisted preparation of nano copper with nano graphene oxide nanocomposite and evaluation of its anti-inflammatory and antioxidant properties. Material and methods: Anti-inflammatory and antioxidant properties of the nanocomposite were assessed using Bovine Serum Albumin (BSA) and DPPH Assay respectively at 10 µL, 20 µL, 30 µL, 40µL, 50 µL. Results: Values for anti-inflammatory property of nanoparticles were higher than the standard values at 40µL, 50 µL concentrations. Percentage of inhibition was highest at 40 µL (86%) and 50 µL (84.6%). The values for antioxidant property of nanoparticles were found to be higher than the standard values at concentrations except at 40µL, 50 µL. Percentage of inhibition was highest at 20 µL (86.2%). Conclusion: Within the limits of the study, it can be concluded that copper and graphene oxide nanocomposite has exceptional anti-inflammatory and antioxidant properties and further can be incorporated in dental material or can be used to coat suture materials to improve their properties.