Preparation and Optimization of Cellulose Nanofibers for Controlled Drug Delivery in Regenerative Endodontics
Author(s): Meshram Rishikesh K*, Hegde Vibha R, Daryapurkar A and Sathawane N
Abstract
Nowadays, search for different drug delivery strategies is going on in endodontics to preserve the vitality of stem cells for regeneration. Cellulose, a linear polymer composed of glucose units, is the most abundant of all naturally occurring organic materials. Electrospun Cellulose Nanofibers (ECN) is being used in medical field for targeted drug delivery in cancer patients. ECN is a promising option and can be a great source of constant drug delivery for disinfection and dental stem cell survival in regenerative endodontics. Hence the purpose of this study was to optimize the different electrospinning parameters to prepare cellulose nanofibers for using it as a drug delivery agent in regenerative endodontics. In this study, Cellulose Acetate (CA) nanofibers were electros punned using Tri Fluoroacetic Acid (TFA) by varying the concentrations, voltage and flow rate while keeping the Tip to Collector Distance (TCD) constant. Three different concentrations 9wt%, 11wt% and 13wt% polymer solutions were prepared and tested for NF formation. Field emission scanning electron microscopy (FESEM) was done to find out the percentage of NF. The results of the present study showed that 11% concentration at 26.5 KV and at 0.1ml/hr produces maximum amount of cellulose NF intermingling with each other as compared to 9wt% and 13wt%.