Prevalence & antibiogram of Pseudomonas aeruginosa at S.S.G. Hospital, Baroda, Gujarat, India
Author(s): Jignasha Tadvi, T. B. Javadekar, Rachana Bhavsar, Nirav Garala
Background: Pseudomonas aeruginosa is a gram negative bacterium that continues to be a major cause of opportunistic nosocomial infections, causing around 9-10% of hospital infections. It is hard to treat because of intrinsic resistance of the species and its ability to further resistance to multiple groups including β-lactams, aminoglycosides and fluoroquinolones.
Aims: This study was undertaken to determine the prevalence of Pseudomonas and their susceptibility pattern at S.S.G. Hospital, BARODA.
Materials and Methods: Between March 2015 to May 2015, 150strains of P. aeruginosa were isolated from different clinical specimens. The samples were selected on the basis of their growth on Mac Conkey and nutrient agar medium with oxidase positive. Colonies were subjected to biochemical tests to identify species. Antimicrobial susceptibility of all the isolates was performed by disc diffusion (Kirby –Bauer) method according to CLSIs guidelines.
Results: Majority of isolates of P. aeruginosa were obtained from specimens of blood, pus, wound, sputum, tracheal aspirates, pleural fluid, ICD fluid, bile fluid.The prevalence of pathogen was 4.15% and 98% pathogens were sensitive Piperacillin+Tazobactum followed by Meropenem (93.33%), Levofloxacin (92.66%) , Ceftazidime (82%), Cefoperazone(81.33%), Piperacillin (80.66%), Amikacin(56%), Gentamicin(54.66%).
Conclusion: The results confirmed the occurrence of drug resistant strains of P. aeruginosa. Meropenem, Levofloxacin and Piperacillin+Tazobactumwere found to be the most effective antimicrobial drugs. It is rational treatment regimens prescription by the physicians to limit the further spread of antimicrobial resistance among the P. aeruginosa strains.