Protective Effect of Asialized Erythropoietin in Modelling of Retinal Ischemia-Reperfusion
Author(s): Kristina V Shchurovskaya, Anna S Pobeda*, Mikhail V Pokrovskii, Nikolai V Solovev, Valentina A Kulikovskaya, Anna A Peresypkina, Vladimir I Shutov, Olesya A Puchenkova and Alexey A Shabalin
An important task of pharmacology is to find effective means to improve the microcirculation of the retina and increase its resistance to ischemia. The aim of the study was to evaluate the retinoprotective effect of asialized erythropoietin on a model of retinal ischemia-reperfusion in rats. The study was performed on a retinal ischemia-reperfusion model, in which intraocular pressure (IOP) was increased to 110 mm Hg for 30 minutes. The retinoprotective effect of asialized erythropoietin at a dose of 0.4 mg/kg and 2.4 mg/kg compared with recombinant erythropoietin at a dose of 50 IU/kg was evaluated by the level of retinal microcirculation using laser Doppler flowmetry (LDF) and electroretinography (ERG) after 72 hours of reperfusion. The use of asialized erythropoietin at doses of 0.4 mg/kg and 2.4 mg/kg resulted in an increase in the level of retinal microcirculation to 718.1 ± 7.1 p.u. and 746.8 ± 9.3 p.u., the b/a coefficient was 2.17 ± 0.09 and 2.41 ± 0.12, respectively, compared with the group without treatment. Thus, asialized erythropoietin can be used as a retinoprotective agent.