The Effects of Nigella sativa Hydro-alcoholic Extract and Honey on Lipid Profile and Indices of Insulin Resistance in Polycystic Ovarian Syndrome Wistar Rat Model
Author(s): Seiyedeh Narjes Naseran, Mokhtar Mokhtari*, Mahmood Abedinzade, Mehrdad Shariati
Background: Polycystic ovary syndrome (PCOS) is a common complex endocrine and metabolic disorder. The cause of PCOS is still unknown and may be due to several factors. The aim of the present study was to evaluate the combined effect of Nigella sativa hydro-alcoholic extract and honey on triglyceride (TG), total cholesterol (TC), high density lipoproteincholesterol (HDL-C) and low density lipoprotein-cholesterol (LDL-C), glucose and insulin in PCOS Wistar rat model.
Materials and Methods: 72 adult Wistar rats, weighting 200-220 g were divided randomly into 9 groups (n=8): including control (intact), Sham (letrozole solvent), control PCOS and 6 experimental PCOS groups. Rats were treated with letrozole for 21 days to induce PCOS. In experimental groups, PCOS rats were treated with 2 doses of honey (1200 and 2400 mg/k), 2 doses of Nigella sativa extract (300 mg/k and 600 mg/k) and 2 doses of combination of Nigella sativa extract and honey by the gavage for 28 days. After harvesting blood serum, lipid profile, glucose and insulin were measured by ELISA method. SPSS software version 16 was used for data analysis.
Results: Serological analysis showed lower TG, TC, LDL, Glucose and insulin in all experimental groups. TG, TC, LDL, Glucose and insulin, particularly at dose 2 of combination of Nigella sativa extract and Honey was significantly decreased (p ≤ 0.05) as compared with control PCOS group. HDL level was significantly increase in dose2 of combination of N. sativa extract and Honey groups.
Conclusion: Combination of Nigella sativa extract and Honey improves lipid profile and the sensitivity to insulin in patients with PCOS.