The Study of Ethanolic and Aqueous Extracts of Plantago psyllium Seed on Pathogenic Multidrug- Resistant Gram Negative Bacteria
Author(s): Zahra Ataeii kachoei, Sima Yahyaabadi, Monir Doudi
Abstract
In recent years, due to the indiscriminate use of synthetic drugs are irrational drug resistance of pathogenic microorganisms is increasing the need for new compounds. The purpose of this study was to investigate the effect of ethanolic and aqueous extracts of Plantago psyllium seed on Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Citrobacter freundii, Enterobacter aerugenes, Pseudomonas aeruginosa and Acinetobacter baumannii, from hospitals (Shariati, Sadoughi, Garazi) and Vahid Medical Laboratory in zarrin shahr were isolated. Also, on the standard of bacteria. Plantago psyllium seed of Agriculture and Natural Resources Research Center of Isfahan was prepared. aqueous and ethanolic extracts were prepared by maceration method. After identification of bacteria, antibiotic resistance was determined by disk diffusion method. Antibacterial effect of ethanolic and aqueous extracts of P. psyllium seed on the growth of multi-drug resistant bacteria by plate diffusion in four ml of 50,100,400 and 800 mg were studied. The MIC and MBC of this extract on bacteria macro dilution method was used. Well diffusion of ethanol and aqueous extract of P. psyllium seed not only mortality but also inhibited the effect on the growth of isolates was observed. The ethanolic and aqueous extract P. psyllium seed neither inhibited nor lethal effect on standard strains of bacteria. The results showed that the aqueous and ethanolic extract P. psyllium seed on multidrug-resistant bacteria (MDR) is not effect antibacterial.
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