The Gas Chromatography Mass Spectroscopy Analysis of One Unani Drug, "Majoon Falasifa"

Hassan Mohammad M¹, Janaki CS², Rao MRK^3*, Prabhu K⁴, Deepa K⁵, Franklin⁶ and Vijayalakshmi N⁷

^*Correspondence: Rao MRK, Department of Anatomy, Amritha University, Thiruporur, Tamil Nadu, India, Email:

Author info »

Introduction

The Unani medicine, Majoon falasifa has been claimed to have properties such as brain and nerve tonic, gastro tonic, as an aphrodiciac and as semen procreator. It is prescribed for dyspepsia, kidney pain and arthralgia also. The ingredients of this medicine are mentioned below:

Tukhm baboona (Anthemis nobilis seed) 15 gm; Amla muqashsher (Emblica officinalis G. crushed fruit) 30 gm; Beikh baboona (Anthemis nobilis root) 30 gm; Filfil daraz (Piper longum fruit) 30 gm; Filfil siyah (Piper nigrum fruit) 30 gm; Maghz chilghoza (Pinus gerardiana kernel) 30 gm; Narjeel taaza (Lodoicea maldivica Pers fresh fruit) 30 gm; Salab misri (Orchis latifolia root) 30 gm; Sheetraj (Plumbago zeylanicum) 30 gm; Zanjabil (Zingiber officinalis rhizome) 30gm; Zaravand mudharij (Aristolochia rotunda root) 30 gm and Maveez munaqqa (Vitis vinifera seed less fruit) 90 gm. All the above ingredients are powdered and mixed with alkaline solution of a particular consistency of Asl (Honey), double the weight of the powder. It is prescribed at dose of 5 to 7 gm.

Very few reports on the medical efficacy of Majoon falasifa are available. Zarnigar have reported the positive role of this medicine in health promotion in elderly patients [1]. Younis et al. have studied the shelf life of Majoon falasifa [2]. Rashid et al. have reported the positive role of Majoon falasifa in the health of elderly [3]. Younis et al. have reported the microbial analysis of this medicine [4]. In order to scientifically evaluate the efficacy of alternative medicines latest techniques the present workers have reported some data and this report is one more step in this direction [5-23].

Materials and Methods

The medicine Majoon falasifa was bought from a Unani medicine vendor. The medicine was suitably processed by standard procedures and the GC-MS analysis was performed.

Results

The Unani medicine Majoon falasifa profile and possible medicinal role of each molecule is tabulated in Table 1.

Table 1: Indicates the retentions values, types of possible compound, their molecular formulae, molecular mass, peak area and their medicinal roles of each compound as shown in the GC MS profile of Majoon falasifa.

Figure 1 shows the GC-MS profile of the Unani medicine Majoon falasifa. The biomolecules were identified by referring to NIST spectral library the possible pharmaceutical roles of each bio molecule as per National Agriculture Library, USA and others as shown in Table 1 [24].

Figure 1: Indicates the gas chromatography mass spectroscopic profile of majoon falasifa.

Majoon falasifa profile showed some important compounds such as, Butanoic acid, 2-methyl-, 1,2-dimethylpropyl ester, Propanoic acid, 2,2-dimethyl-, 2-ethylhexyl ester, 5-Hydroxymethylfurfural, Cyclohexane, 1-ethyl-1,3-dimethyl-, trans-, Gamolenic Acid, Methyl 2-hydroxy-octadeca-9,12,15-trienoate, 1,2-Benzenedicarboxylic acid, mono (2-ethylhexyl) ester etc. the medicinal roles of each is mentioned in Table 1. These medicinal roles correspond to the medicinal role of Majoon falasifa. Further work to understand the medicinal roles of some of the molecules whose roles have not been reported yet will be worthwhile.

Discussion

Majoon falasifa profile showed some important compounds such as, Butanoic acid, 2-methyl-, 1,2-dimethylpropyl ester, Propanoic acid, 2,2-dimethyl-, 2-ethylhexyl ester, 5-Hydroxymethylfurfural, Cyclohexane, 1-ethyl-1,3-dimethyl-, trans-, Gamolenic Acid, Methyl 2-hydroxy-octadeca-9,12,15-trienoate, 1,2-Benzenedicarboxylic acid, mono (2-ethylhexyl) ester etc. the medicinal roles of each is mentioned in Table 1. These medicinal roles correspond to the medicinal role of Majoon falasifa. Further work to understand the medicinal roles of some of the molecules whose roles have not been reported yet will be worthwhile.

Conclusion

It could be summarized from the results and discussion that Majoon Falasifa does contain important biomolecules which provides a clue to its prescription for the urolithiatic role of this medicine. It will be of interest to probe into the medicinal roles of many compound present in Majoon Falasifa for which the medicinal roles are not reported yet.

References

1. Rashid B, Zarnigar. Evaluating the role of Majoone Falasifa (Herbal formulation) in health promotion of elderly: A randomized single blind placebo controlled study. Am J pharm health res 2014; 2:214-231.
2. Younis PM, Rahman K, Rashid B. 'Accelerated Stability study of Majoone Falasifa (A Unani Herbal Formulation'. J bio Sci Opinion 2015; 3187-194.
3. Rashid B, Itrat M, Kha ZA. 'Majoon Falasifa: A Boon from Unani Medicine for Elderly'. J Pharmacol 2016; 6:1-5:193.
4. Younis PM, Rahman K, Rashid B et al. 'Microbial analysis of Majoone Falasifa (A Unani Herbal Formulation' J clin pharmacolmicrobiol Toxicol 2015; 1:32-34.

   [Google Scholar]

5. Rao MRK, S Philip, Kumar MH, et al. GC-MS analysis, antimicrobial, antioxidant activity of an Ayurvedic medicine, Salmali Niryasa. J Chem Pharm Res 2015; 7:131-139.
6. Sivakumaran G, PrabhuK, Rao MRK, et al. Gas chromatography–mass spectrometry analysis of one ayurvedic oil, Anu thailam. DIT 2019; 11:2675-2678.
7. Sivakumaran G, Prabhu K, Rao MRK, et al. Gas chromatography–mass spectrometry analysis of one ayurvedic oil, Ksheerabala Thailam. DIT 2019; 11: 2661-2665.
8. Sivakumaran G, Prabhu K, Rao MRK, et al. Gas chromatography–mass spectrometry analysis of one Ayurvedic oil, Triphaladi Thailam. DIT 2019; 11:2679-2683.
9. Narayanan G, Prabhu K, Rao MRK, et al. Gas chromatography–mass spectrometry analysis of one Ayurvedic medicine, Drakshadi Kashayam. DIT 2019; 11: 2652-2656.

   [Google Scholar]

10. Narayanan G, Prabhu K, Rao MRK, et al. Gas chromatography–mass spectrometry analysis of one ayurvedic medicine, Kutajarishtam. DIT 2019; 11:2666-2669.
11. Narayanan G, Prabhu K, Rao MRK, et al. Gas chromatography–mass spectrometry analysis of one Ayurvedic antiobesity medicine, Lohasava. DIT 2019; 11:2670-2674.
12. Kumar MH, Prabhu K, Rao MRK, et al. Gas chromatography/mass spectrometry analysis of one Ayurvedic skin oil, Eladi Kera Thailam. DIT 2019; 11:2657-2660.
13. Mohammad H, Prabhu K, Rao MRK, et al. The GC MS study of one Ayurvedic Pain relieving OIL "Mahamasha thailam". Drug Discov Today 2019; 12:1524-1527.
14. Mohammad H, Prabhu K, Rao MRK, et al. The GC MS study of one Ayurvedic Pain relieving oil "Karpooradi thailam", Drug Invention Today, 2019; 12:1542-1546.
15. Prabhu J, Prabhu K, Chaudhury A, et al. Neuro protective role of Saraswatharishtam on Scopolamine induced memory impairment in animal model. Pharmacogn J 2020; 12:465-472.

    [Crossref][Google Scholar]

16. Prabhu K, Rao MRK, AK Bharath, et al. The GC MS study of one Ayurvedic Rasayana formulation Narasimha Rasayanam. DIT 2020; 13:658-662.
17. Prabhu K, Rao MRK, Vishal S K, et al. GC MS study of one Ayurvedic Rasayana drug, Dhanwantari Rasayanam. DIT 2020; 14:783-786.
18. Sharmila D, Poovarasan A, E Pradeep, et al. GC MS analysis of one Ayurvedic formulation, Sitopaladi. RJPT 2021; 14:911-915.
19. Narayanan G, PrabhuK, Chaudhuri A, et al. Cardio protective role of Partharishtam on isoproterenol induced myocardial infarction in animal model. Pharmacogn J 2021; 13:591-595.

    [Crossref][Google Scholar]

20. Kalivannan J, Janaki CS, Rao MRK, et al. The GC MS astudy of one ayurvedic formulation, Chandanasavam. Ind J Nat Sci 2021; 12:33671-33676.
21. Akshaya SR, Kalaivani S, Prabhu K, et al. The GC MS study of one Ayurvedic churnam, Avalgujabijadi churnam. Ind J Nat Sci 2021; 12:34395-34402.
22. Subbiah AJ, Kavimani M, Rao MRK, et al. The GC MS study of one Ayurvedic. Formulation, Pushyanuga churnam. Ind J Nat Sci 2021; 12:35757-357-366.

    [Crossref][Google Scholar]

23. Yuvaraj R, Vijayakumar S, Rao M R K et al. The GC MS study of one Ayurvedic medicine Pippalyasavam'. Ind J Nat Sci 2021; 12:35612-35618.
24. Duke, James A. Duke's Phytochemcial and Ehnobotanical Databases. U.S. Department of Agriculture, Agricultural Research Service. Ag Data Commons, U.S, 2021, 183.

    [Crossref] [Google Scholar]