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The Gas Chromatography Mass Spectroscopy Analysis of One Unani Drug, "Sherbath E-Bailphal"

Journal of Research in Medical and Dental Science
eISSN No. 2347-2367 pISSN No. 2347-2545

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Research Article - (2022) Volume 10, Issue 9

The Gas Chromatography Mass Spectroscopy Analysis of One Unani Drug, "Sherbath E-Bailphal"

Hassan mohammad M1, Janaki CS2, Rao MRK3*, Prabhu K4, Deepa K5, Franklin6 and Vijayalakshmi N7

*Correspondence: Rao MRK, Department of Anatomy, Amritha University, Thiruporur, Tamil Nadu, India, Email:

Author info »

Abstract

The Unani medicine, Sherbath e-bailphal a health drink made of the pulp of Bail phal, or Bilva (Aegle marmelos L). This fruit has many medicinal values and can be taken as a health drink. The medicine was bought from a Unani medicine supplier and was processed suitably to be analysed by GC MS process. The pofile showed many molecules, namely, 8-Hydroxy-2, 2, 8-trimethyldeca-5,9-dien-3-one, 5-Hydroxymethylfurfural, 1,15-Pentadecanedioic acid, Methyl 2-hydroxyoctadeca-9,12,15-trienoate, Methyl 3-cis,9-cis,12-cis-octadecatrienoate, (E,Z,Z)-2,4,7-Tridecatrienal, Hexadecanoic acid, cyclohexyl ester etc. which could play the medicinal roles of Sherbath Ibilphal.

Keywords

GC MS, Unani, Sherbath e-bailphal, 8-Hydroxy-2, 2, 8-trimethyldeca-5,9-dien-3-one, 5-Hydroxymethylfurfural, 1,15-Pentadecanedioic acid

Introduction

Sherbath e bailphal is the health drink made of the pulp of Bail phal, or Bilva (Aegle marmelosL).Bilva has many medicinal roles [1]. Sherbath e-bailphal is taken as health drink particularly during summer as a coolant. The present workers have worked to scientifically evaluate the veracity of these medicine systems by latest techniques so that deeper knowledge of the mechanism of action of these medicines could be gained [2-20]. The present study in one step further in this endeavour. Not much work in this direction is reported as far as “Sherbath e-bailphal” is concerned.

Materials and Methods

The medicine Sherbath e-bailphal was bought from Unani medicine supplier and was suitably processed by standard procedures and the GC-MS analysis was performed.

Results

The gas chromatography mass spectroscopic profile of Sherbath e-bailphal and possible medicinal role of each molecule is tabulated in Table 1.

Ret. Time Molecule Mol. Formula Mol. Mass % peak Area Possible Medicinal Role
3.75 8-Hydroxy-2,2,8-trimethyldeca-5,9-dien-3-one C13H22O2 210.2 0.23 17 beta hydroxysteroid dehydrogenase inhibitor
4.33 10-Chlorodecyl isobutyl carbonate C15H29ClO3 292.2 0.24 Not known
4.81 Benzene, 1,3-bis(1,1-dimethylethyl)- C14H22 190.2 1.76 Not known
6.31 5-Hydroxymethylfurfural C6H6O3 126 4.08 It is reported to stop neuron apoptosis
8.03 Methylparaben C8H8O3 152 19.3 Not known
10.79 13-Tetradecenal C14H26O 210.2 0.24 Not known
13.78 Palmitoyl chloride C16H31ClO 274.2 0.82 Not known
14.29 1,15-Pentadecanedioic acid C15H28O4 272.2 2.61 Arachidonic acid-Inhibitor, Increase Aromatic Amino Acid Decarboxylase Activity, Inhibit Production of Uric Acid,
16.82 9-Octadecenoic acid, (E)- C18H34O2 282.3 12.37 Not Known
17.06 Methyl 2-hydroxy-octadeca-9,12,15-trienoate C19H32O3 308.2 12.8 17 beta hydroxysteroid dehydrogenase inhibitor, aryl hydrocarbon hydroxylase inhibitor, testosterone hydroxylase inducer, Catechol-O-Methyl-Transferase-Inhibitor
17.5 Methyl 3-cis,9-cis,12-cis-octadecatrienoate C19H32O2 292.2 0.21 Catechol-O-Methyl-Transferase-Inhibitor, Methyl-Donor, Methyl-Guanidine-Inhibitor
17.75 (E,Z,Z)-2,4,7-Tridecatrienal C13H20O 192.2 0.52 Provides zinc, anticancer, antidote, Cancer-Preventive, Cytochrome-P450-2E1-Inhibitor, Decreases C telopeptide excretion, decrease deoxypyridinoline excreation, decreses endothelial leukocyte adhesion, decrease endothelial platelet adhesion, decrease epinephrine production, decreases oxalate excretion.
29.39 Hexadecanoic acid, cyclohexyl ester C22H42O2 338.3 37.16 Increase Aromatic Amino Acid Decarboxylase Activity, Inhibit Production of Uric Acid

Table 1: Indicates the retentions values, types of possible compound, their molecular formulae, molecular mass, peak area and their medicinal roles of each compound as shown in the GC MS profile of Sherbath e bailphal.

Figure 1 depicts gas chromatography mass spectroscopic profile of Sherbath e-bailphal. The metabolites were identified by comparison with NIST spectral library and the possible pharmaceutical roles of each bio molecule as per National Agriculture Library, USA and others as shown in Table 1 [21].

medical-dental-science

Figure 1: Shows the gas chromatography mass spectroscopic profile of Sherbath e bailphal.

Discussion

The gas chromatography mass spectroscopic profile of Sherbath e-bailphal shows compounds, namely, 8-Hydroxy-2, 2, 8-trimethyldeca-5,9-dien-3-one, 5-Hydroxymethylfurfural, 1,15-Pentadecanedioic acid, Methyl 2-hydroxy-octadeca-9,12,15-trienoate, Methyl 3-cis,9-cis,12-cis-octadecatrienoate, (E,Z,Z)-2,4,7-Tridecatrienal, Hexadecanoic acid, cyclohexyl ester etc. which have medicinal roles, as shown in Table 1. These molecules could be contributing to the cure of diseases for which Sherbath e bailphal is prescribed.

Conclusion

It could be summarized from the results and discussion that Sherbath e-Bailphal does contain important biomolecules which provides a clue to its prescription for the ailments it is given. Some of the molecules shown in the GC MS profile for which the medicinal roles are not known could be further probed.

Acknowledgements

The authors thankfully acknowledge the support of all the people and organizations.

References

Author Info

Hassan mohammad M1, Janaki CS2, Rao MRK3*, Prabhu K4, Deepa K5, Franklin6 and Vijayalakshmi N7

1Department of Anatomy, Northern Borders University, Arar, Saudi Arabia
2Department of Anatomy, Bhaarath Medical College, Chennai, Tamilnadu, India
3Department of Anatomy, Amritha University, Thiruporur, Tamil Nadu, India
4Department of Anatomy, Sree Balaji Medical College and Hospital, Chennai, Tamil Nadu, India
5Department of Anatomy, Quest International University, IPOH Perak, Malaysia
6Dept of Microbiology, CEO Anna Medical College, Mauritius, Montagne Blanche, Island, Mauritius
7Department of Chemical and Biotechnology, SASTRA (Deemed to be University), Thanjavur, Tamil Nadu, India
 

Received: 02-Jul-2022, Manuscript No. JRMDS-22-57369; , Pre QC No. JRMDS-22-57369; Editor assigned: 05-Jul-2022, Pre QC No. JRMDS-22-57369; Reviewed: 19-Jul-2022, QC No. JRMDS-22-57369; Revised: 02-Sep-2022, Manuscript No. JRMDS-22-57369; Published: 08-Sep-2022

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