Antioxidant Activity of Silver Nanoparticles Synthesized Using Vetiveria zizanioides-In Vitro Study
Introduction: Nanotechnology has a wide range of biomedical applications and nanoparticles can be devised to possess unique composition and functionalities, which can provide novel tools and techniques in biomedical research. Nanotechnology is one of the rapidly developing sciences in the past few years. Nanoparticles are materials that have all their dimensions in the Nano scale and measured in nanometres. The size-dependent physicochemical properties of nanoparticles promote their application in many products but the same unique properties also can lead to physiological responses in living systems by interaction with these materials. Aim: The aim of the present study is to synthesize silver nanoparticles from aqueous extract of Vetiveria zizanioides plant and to evaluate its antioxidant potential. Materials and methods: Plant extract of Vetiveria zizanioides was prepared and filtered by Whatman No 1 filter paper. Silver nitrate was added to the plant extract and kept in a magnetic stirrer for nanoparticle synthesis. The synthesized nanoparticle was preliminarily analysed using UV visible spectroscopy. Finally the left over solution was taken to calculate antioxidant activity. Results and discussion: Antioxidant activity was calculated by DPPH method and the percentage of inhibition of silver nanoparticles synthesised from Vetiveria zizanioides was 46.9% for 10µL, 56.9% for 20µL, 61.5% for 30µL, 67.3% for 40µL and 72.4% for 50µL. Hence maximum inhibition was observed at 50µL that is at higher concentration Conclusion: We can conclude that silver nanoparticles synthesised from Vetiveria zizanioides are a potent antioxidant agent. Since it shows a good activity in free radical scavenging, silver nanoparticles can be used in a clinical therapeutic application.