Assessment of Cytotoxicity of Copper and Graphene Oxide Nano composite Synthesized Using Amla Extract Formulation-An In-Vitro Study
Background: Several conventional methods are used for synthesis of nanoparticles. But toxic chemicals are required as capping agents to maintain stability, thus leading to toxicity in the environment. Thus, we need to shift to “Green Synthesis”. Hence, this study was conducted to assess the cytotoxicity of copper and graphene oxide nano composite reinforced with amla extract. Copper is well known for its intrinsic antibacterial properties, graphene oxide for its barrier and structural strength hence these were chosen to create a nanocomposite. Aim: Aim of the study was green preparation of nano copper with nano graphene oxide nanocomposite and its cytotoxicity evaluation. Material and methods: Cytotoxic effect of copper and graphene oxide nanocomposite with amla extract was assessed using Brine Shrimp Assay respectively at 5 µl, 10 µl, 20 µl, 40 µl, 80 µl. Results: The copper and graphene oxide nanocomposite are safe to be used in the dental materials till 20 µL concentration. Although at 40 µl, 80 µl concentrations cytotoxicity was observed. Conclusion: Within the limits of the study, it can be concluded that copper and graphene oxide Nano composite can be safely used in concentrations up to 20 µL as periodontal dressing or incorporated as bone grafts in periodontal regeneration.