Comparative Study Between Three Different Types of Denture Base Material for the Ability to form the Biofilm of Bacteria
Author(s): Mohmmed R, Karama T Al-Taee*, Anas Hammad Abed
Aim: The aim of this study was to compare between three different types of denture base material heat cure acrylic resin (conventional acrylic), flexible acrylic and compact acrylic (acrylic block) according to the ability of their surfaces for bacterial adhesion and biofilm formation to know their susceptibility for contamination during dentures placement.
Materials and Methods: In our study, we were using two types of bacteria Pseudomonas aeruginosa and Staphylococcus epidermidis. That was previously tested for their ability to produce biofilm. To detect and measure the formation of biofilm, Quantitative biofilm assay and Scanning Electron Microscope were used.
Results: According to the results of (Quantitative) biofilm assay, the surfaces of the three dental materials showed significant differences in their ability to form biofilm. The results adhesion of P. aeruginosa and S. epidermidis on the three surfaces showed that, the conventional acrylic surface has the most ability to accumulate these bacteria and forming biofilm after contamination and incubation 24 hours, while the compact acrylic (block) showed less ability to forming biofilm, but the flexible acrylic was in between them.
Conclusion: Compact acrylic (acrylic block) in construction of dentures by the CAD-CAM technology has the advantage of decreasing the accumulation of bacteria (P. aeruginosa and S. epidermidis) on the surface of dentures in comparison with using the conventional acrylic and flexible acrylic by the conventional method (flasking procedure). In addition to that the P. aeruginosa was more prevalent than the S. epidermidis on the surfaces of the samples.