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Comparing the Antimicrobial Activity of Catgut Suture Coated with Neem, Ginger and Mint Infused In Silver Nanoparticles-An in-Vitro Study | Abstract

Journal of Research in Medical and Dental Science
eISSN No. 2347-2367 pISSN No. 2347-2545

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Comparing the Antimicrobial Activity of Catgut Suture Coated with Neem, Ginger and Mint Infused In Silver Nanoparticles-An in-Vitro Study

Author(s): Guy Patrick Sandou1*, Subhashree R2, S Rajeshkumar3, Thiyaneswaran Nesappan4

Abstract

Introduction: Plants play an important part in drug production, and the pharmaceutical industry relies extensively on
natural ingredients to produce new drugs. According to a WHO survey, folk medicine is used by 80% of the world's
population for primary health care. Nanoparticles have attracted a lot of attention due to their special properties and
potential applications due to their large surface area and surface plasmon resonance effect.
Aim: The aim of this study was to evaluate the antimicrobial effect of green synthesis of neem, ginger and mint infused in
silver nanoparticles.
Methodology: Neem, ginger and mint were washed thoroughly and shade dried. The dried plant material was finely
powdered and grounded using a slender blender. An aqua extract of neem, ginger and mint was prepared separately. 50 ml
of silver nanoparticles were mixed with a 50 ml solution of aqua extract. Kept in a shaker and UV-vis Spectrophotometer
readings are taken at 12,24,48 hrs to check for the formation of nanoparticles. UV-Visible spectrophotometry was used to
classify the synthesized silver nanoparticles, revealing peak absorption at 440 nm and a composite at 380 nm. The uniformly
scattered spherical shaped silver nano-composite with a size of about 14-20 nm was clearly visible in the TEM pictures. The
biosynthesized nanocomposite was coated on catgut suture material to test its anti-peri-implantitis effect. In the in-vitro
study, the antimicrobial activity of the three plants was analysed by agar well method using organisms Lactobacillus,
Streptococcus mutans and Candida albicans. Results: Preventing any post-surgical infection is an important parameter for obtaining the desired outcome. This study
showed that neem, ginger, mint Ag coated sutures showed an effective antimicrobial action against L. bacilli, S. mutans and
C. albicans, which are the most commonly found oral microbiota.
Conclusion: Ginger Ag+chitosan nanocomposite showed a significant potent effect against E. fecalis, S. mutans,
Pseudomonas, C. albicans.

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