Development, Characterization and Evaluation of Nano Formulation of Abrus Precatorius Root Extract for Anti Inflammatory Activity
Author(s): Sneha Nawale*, Sindhu Bhargavi M, Prasanna Laxmi K and Ganga Raju M
Abstract
The aim of the present study was to develop nano formulation of Abrus precatorius root extract (NE-AP) and characterize for particle size, poly dispersed index (PDI), entrapment efficiency, zeta potential, SEM, XRD and differential scanning microscopy (DSC). NE-AP was assessed for its anti-inflammatory potential with carrageenan induced rat paw edema, formalin induced rat paw edema and cotton pellet granuloma in rats. Average particle size of NE-AP was found 35 nm, PDI 60%, entrapment efficiency 46%, zeta potential -23mV. XRD showed the characteristic peaks at 32, 41 and 67 θ. SEM revealed the spherical shape and smooth surface of particle, and in DSC melting endotherm was observed at 329.37º C. NE-AP (50 mg/kg bd.wt and 100 mg/kg bd.wt) showed significant antiinflammatory activity with carrageenan induced inflammation (p˂0.01, p˂0.05), formalin induced inflammation (p˂0.01, p˂0.05), and cotton pellet granuloma (p˂0.01, p˂0.05) when results were compared with standard and disease control animals. Nanoemulsion of Abrus precatorius [NE-AP] showed prominent anti-inflammatory activity so can be used for management of anti-inflammatory activity with improved permeability.