Effect of Lupeol on Pro-Inflammatory Markers in Adipose Tissue of High-Fat Diet and Sucrose Induced Type-2 Diabetic Rats
Background: Lupeol also called Fagarsterol is found in white cabbage, green pepper and strawberry. It is reported to have beneficial effects for various disorders. However, the mechanisms by which lupeol controls proinflammation-mediated signalling is obscure. Aim: The study was aimed at assessing the effect of lupeol on the expression of proinflammatory signalling molecules antidiabetic activity of lupeol through the expression of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in adipose tissue of high fat diet-induced type-2 experimental rats. Materials and methods: Male Albino Wistar rats were used for the present study and divided into 4 groups of 6 animals each. Group 1: Served as normal control rats; Group II: Serves as high fat diet-induced type-2 diabetic rats; Group III: Diabetic rats treated with Lupeol (25 mg/kg b.wt) and Group IV: Diabetic administered treated with metformin. At the end of the treatment, adipose tissues from control and treated animals were dissected out and used for the assessment of mRNA expression of proinflammatory cytokines such as IL6 and TNF-α. The data were statistically analysed using one-way-ANOVA to check the statistical significance between groups. Results: The IL6 and TNF-α mRNA expression levels were measured using RT-PCR method. The results showed that the IL6 and TNF-α mRNA expression level of diabetic rats were significantly increased (p<0.05) whereas on the treatment with lupeol the levels were near normal which were also compared with standard drugs. Conclusion: The present findings show that lupeol has a significant role in controlling the expression of proinflammatory signalling molecules in adipose tissue and hence it may be considered for the development of new antidiabetic therapeutic drugs.