Virulence genes and genomic sequence of A. baumannii from different clinical samples
125 MDR clinical isolates of Acinetobacter baumannii were tested for ESBL, carbapenemases and MBL production using standard protocol. 59 were ESBL and 79 were Carbapenemase and MBL producing Acinetobacter baumannii isolates. The genotypic expression of the virulence factors were evaluated for 15 MDRAB (Multi Drug Resistant Acinetobacter Baumannii) and 15 susceptible isolates using conventional multiplex Polymerase Chain reaction. There was statistically significant association between MDRAB and susceptible AB in respect to carbapenamase gene (p=<1.000) and MBL gene (P=0.002). The most relevant finding of this study was the identification of blaOXA 51 gene in one of the 15 susceptible isolates was isolated. This indicates the need for testing of even susceptible isolates for virulence factors. 5 MDRAB isolates were selected for genome sequencing and it was 100% confirmed as Acinetobacter baumannii at species level. This present study has brought out the importance of virulence testing for all AB isolates by genotypic methods.