Stephen Selvaraj
Department of Microbiology, Mahatma Gandhi Medical College & Research Institute, Sri Balaji Vidyapeeth (Deemed-to-be-University), Pondicherry, IndiaPublications
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Original Article
Isolation of Diverse Mycobacterium Tuberculosis Strains Employing Automated and Conventional Culture from Lymphadenitis in a Tertiary Care Center, Pondicherry, India
Author(s): Kandhakumari Gandhi, Stephen Selvaraj* and Kavitha Kannaiyan
In India, tuberculosis remains a major public health problem and Tuberculous lymphadenitis remains a diagnostic challenge. This study aimed to isolate M. tuberculosis from lymphadenitis employing LJ and MGIT culture. A total of 49 single clinical specimens collected from patients with clinical suspicion of Tuberculous lymphadenitis were included. Ziehl- Neelsen microscopy and culture was performed and growth of M. tuberculosis was identified using standard tests. Drug susceptibility testing was carried out in MGIT and the isolated M. tuberculosis strains causing lymphadenitis were genotyped by spoligotyping. Among 49 samples processed, 28.6% were positive by ZN, 20.4% on LJ and 26.7% in MGIT. LJ and MGIT together yield 32.7% positivity. The mean and median turnaround time for LJ and MGIT culture was 31.8 and 14.5 days respectively. Conventional identification tests identified 15 M. tu.. Read More»