Anatomical Abnormalities Caused by Induced Infections in Femur and Tibia of Rattus Norvegicus and the Efficacy of Marine-Derived Antimicrobial Peptides (AMPS) in Treatment
Author(s): Mohammed H Karrar Alsharif*, Muhammad Musthafa, Nagi Mahmoud Bakhit, Elmutasim O Ibnouf, Abubaker Y Elamin, Juman M Almasaad, Khalid M Taha, Abair Awadalla Ahmed Mahdi, M Suliman, Mamoun A Alfaki, Hala M Hamour
This study aimed to assess the impact of gill extraction of antimicrobial peptides which are derived from the marine organism Perna Viridis against anatomical abnormalities. Those have been developed due to induced bacterial infection in the tibia and femur of Rattus norvegicus. Such infections are maintained for three weeks. Methods and Results: Experimental infection was induced in 2 Albino rats. A 109CFU/ml suspension of Staphylococcus aureus and streptococcus pyogenes was injected into the tibia and femur of the experimental rat. On observation of two rats, the clinical symptoms like inflammation, tumours, and histopathological changes. Among histopathological changes mostly inflammatory cells like monocytes, T-lymphocytes, neutrophils were observed more than basophils over the tissue in "Staphylococcus aureus (S.A.)" and "streptococcus pyogenes (S.P.)" infected rat after post-inoculation of infection. Antimicrobial peptides were utilized to treat these anatomical abnormalities. The Culture sensitivity test was done. There was no minimal inhibition concentration observed. Conclusion: Antimicrobial peptides have been demonstrated to kill both gram-negative bacteria and gram-positive bacteria and enveloped. After induction of both bacterial strains into experimental rats, anatomical abnormalities were observed. However, antimicrobial peptides were not shown any efficacy against both bacterial strains.