Are new bioactive materials less harmful to pulp? Real-Time Cytotoxicity Assessment in Vitro
Author(s): Turkay Kaykolus*
Introduction: The aim of this study was to evaluate the cytotoxicity of contemporary restorative bioactive materials and glass ionomers.
Materials and methods: Fuji IX GP Capsule (GC), EQUIA Forte (GC), Biodentine (Septodont), Glass Fill (GCP Dental) and Activa BioActive Restorative (Pulpdent) material specimen’s regional toxicity determined by XTT assay and real-time cell analysis method. For statistical one-way ANOVA, post hoc Tukey's HSD, hierarchical clustering and distance correlation methods used.
Results: Activa BioActive Restorative showed the least cytotoxicity in both experiments. In XTT Glass Fill, in RTCA Fuji IX GP Capsule was found to be the most cytotoxic material. Also, undiluted concentrations of all materials were found to have cytotoxic effects on L929 cells.
Conclusion: Within the limitations of this study, we can say that restorative bioactive materials are less cytotoxic than glass ionomers.