Comparison of Antibacterial Efficacy of Annona Squamosa Mouthwash with Chlorhexidine for Children
Background: Controlling dental plaque is critical in the prevention and treatment of periodontal disease and dental caries. In addition to mechanical plaque removal techniques such as tooth brushing and flossing, the use of chemical and herbal mouthwashes is recommended because it is safe, effective and economic. Natural materials are increasingly being used in dentistry. Aim: This study will be conducted to investigate the antibacterial activity of Annona squamosa pulp mouthwash against Staphylococcus aureus and Escherichia coli in comparison with chlorhexidine. Materials and Methods: This microbiological in vitro study was conducted in the College of Dentistry/ University of Baghdad. Saliva samples were taken from children aged (7-9) years with the same socioeconomic and oral hygiene conditions, healthy child without any history of systemic medical condition. Saliva sample were obtained from the children and taken immediately to laboratory for culturing on Mannitol salt agar and Macconkey agar and incubated aerobically for 24 hours at 37 C. Following their identification and after complete extraction of annona squamosa fruits, the bacteria were plated in Mueller-Hinton agar, and the antibacterial activity of the pulp extracts was assessed using the agar-well diffusion technique. Results: A. squamosa extract have effective inhibitory concentrations against tested bacteria except in concentration 0.5 mg/ml have no effect on E. coli, and inhibition zone increase when concentration increased until 10 mg/ml have highest inhibition effect when comparable with chlorhexidine. Conclusion: A. Squamosa extract is effective as chlorhexidine mouthwash for children.