Evaluating Cytotoxic Effects of Resin Based CAD/CAM Blocks
Background: The effects of CAD/CAM blocks containing resin on oral cells, which are widely used in the restoration of teeth recently, are unknown. The aim of this study is in vitro examination of cytotoxic effect of resin and ZLS (zirconia-reinforced lithium silicate) including CAD/CAM blocks on human gingival keratinocyte (HGK) cells.
Material and Methods: In our study; samples were prepared (1.5 × 7 × 12 mm) under water cooling, using CAD/CAM blocks. Both surfaces of the prepared samples were polished using a diamond polishing kit (Clearfil Twist Dia, Kururay). Samples, whose surface (3 cm2/ml) were calculated according to the International Standards Organization (ISO 10993-12: 2012), were left for 1, 3- and 7-days incubation in DMEM. Cell viability of extracts of 1:1 ratio of filtered CAD/CAM blocks were examined by MTT test. Cell viability results were evaluated using one-way analysis of variance (ANOVA) test (p<0.05).
Results: There was no statistically significant difference between the cell viability values of the extracts of CAD/CAM blocks after 1 and 3 days and the control group (p>0.05). Statistically significant differences were observed in the cell viability values of the extracts of composite resin and ZLS CAD/CAM blocks at the end of 7 days compared to the control group (p<0.05).
Conclusion: Although the extracts of CAD/CAM blocks after 7 days caused a decrease in the viability of HGK cells, they showed cell viability above the cell viability rate (>70%) stated in ISO standards.