Histological Evaluation of Laser Incision Properties Assisted by Exogenous Chromophores: (An in vitro Animal Study)
The diode laser is becoming widely used for oral soft tissue procedures, due to approximate relation of infra- red wavelengths with absorption of oral soft tissue pigments. Thermal damage is one of the drawbacks that result from heating the tissue by laser. The aim of this study is to evaluate histologically the incisions properties of oral soft tissue created by diode 940 nm laser in combination with exogenous stain. Materials and methods: 60 samples of dimensions (1.5 × 1 × 0.5) cm from three sheep tongues collected directly after animal sacrificing divided into two groups. Group I, 20 samples without stain, Group II, 40 samples stained with erythrosine stain 3 %. The incisions made by 940 nm diode laser, with power 2 W in continuous mode. The incision time was recorded for all incisions. For histological examination the samples collected in sterile containers with buffered formalin 10% to evaluate the depth and width of incisions and of lateral damage, the regularity and quality of incisions margin. Result: The group II shows the highest quality incisions, decrease in lateral damage both vertically and horizontally, decrease in the incision time than with group II. Conclusion: using exogenous chromophores can assist producing incision with more regular margin and minimize thermal tissue damage.