Role of Immunohistochemistry Versus Hematoxylin and Eosin and Special Stains in Helicobacter Pylori Detection in Gastric Lesions
Author(s): Ayushi Narain and Ponnuswamy Karkuzhali*
Helicobacter pylori can be demonstrated in tissue by Gram stain, Giemsa stain, haematoxylin & eosin stain, Warthin-Starry silver stain, acridine orange stain, and phase-contrast microscopy. Hence the present study was premeditated to analyse the role of Immunohistochemistry versus Hematoxylin and Eosin and special stains in detection of Helicobacter pylori in gastric lesions. A total of 455 gastric biopsy specimens were received over a period of 2 years from October 2015 to September 2017. Of these 100 cases were selected which had adequate data. 60 cases were randomly selected and analysed for Helicobacter pylori infection using H&E, Giemsa and IHC. The most associated endoscopic finding with gastritis in the present study was erosions followed by erythema (linear bands/erythematous mucosa). Sensitivity and specificity of Giemsa was 95.2 % and 100% respectively. Sensitivity and specificity of H&E was found to be 90.5 % and 100% respectively. Best results were obtained by Immunohistochemistry, especially when the density of organ ism was low. The cost, applicability and authenticity of the Giemsa stain make it an ideal stain in detecting Helicobacter pylori infection in gastric biopsies.